Cytology: Cells are removed from the body, then stained and examined under a microscopy. The trained physician or cytotechnologist
is able to detect the presence of malignancy.
Use of either the 5.0 um or 8.0 um polycarbonate membrane.
Pore Structure and Porosity
Minimum clogging by red blood cells and protein. Well preserved cellular morphology.
High recovery rate. Rapid filtration with low pressure. Surface capture. No cover slip edge sealing.
Smooth, Flat Surface
High cell visibility. Improved morphologic resolution. Surface capture.
Easy mounting. Immediate microscopic examination.
Low absorption and adsorption
proved contrast. Greater cell isolation. Easy mounting.
Improved contrast. Simpler microscopic analysis routine.
Simpler microscopic analysis routine.
Unaffected by conventional cytologic fixatives and stains.
Less critical handling techniques needed.
25mm or 47mm filter holder
Stainless steel forceps
5.0um - 25mm or 47mm PCTE membranes
General Procedure - procedure may vary
Most body fluids contain blood in various amounts.
Collect them in a container with an anticoagulant.
One recommended using polycarbonate membranes is EDTA (0.1 molar)
For each 5-ml specimen generally 1 ml of EDTA solution is sufficient; for specimens of higher blood content, 1 ml of EDTA for each 20-ml specimen is recommended.
Fixatives or preservatives recommended are:
30 to 50% alchol
10% buffered formalin
For optimeum results employ a limited amount of fluid when preparing specimens that exhibit high cell concentrations. If too much fluid is
used with cellular specimens, such as endometrial washing, ascites, gastrics, pleurals, sputums, and urines, the cells crowd and clog
the filter. A 5-ml specimen or smaller volume provides a good sample if it is properly mixed before filtration.
The 8um pore size is recommended for filtering most body fluids.
For samples of higher probability of small cancer cells or fewer cancer cells, such as CSF or baby
urine, the 5um pore size is recommended.
um = micrometer = micron